The biosensor chip, where every inQuiQ measurement happens

Q: What is the difference between the HC1000M and HC30M sensor chip?

Both chips carry a polycarboxylate hydrogel over the photonic sensor. For users familiar with Biacore sensor chips, the practical distinction is similar: the surface is selected according to the measurement. The HC1000M uses long chains and suits low molecular weight analytes, quantification, screening, and specificity panels. The HC30M uses short chains and suits affinity determination and full kinetic characterization. Many programmes screen on one and confirm on the other.

Q: How many targets can one biosensor chip measure?

Each chip has four channels with four sensors, 16 in total. Immobilized in flow, 4 different ligands can be immobilized on the chip. With a microarray spotter the sensors are addressed individually, giving up to 16 ligands for one measurement, including controls and reference surfaces.

Q: Can inQuiQ sensor chips be regenerated and reused like SPR sensor chips?

Yes. The hydrogel surface is regenerable. After each injection the bound analyte is stripped and the surface returns to baseline, so one chip carries a full concentration series and keeps the same immobilized target in place across a measurement series. Anyone used to regenerating an SPR sensor chip will recognize the workflow. Some immobilization techniques, such as Protein A/G, also allow the ligand to be stripped, enabling different ligands to be immobilized and measured sequentially on the same biosensor.

Q: Can unpurified samples be run on these chips?

Yes. The antifouling hydrogel is built for serum, plasma, cell culture supernatant, and other complex matrices, where background binding usually limits the measurement. Filter the sample through a 0.22 µm filter and inject it directly. No purification step is needed first.

Q: Is an NTA sensor chip available for his-tagged ligands?

Yes. His-tagged ligands are captured on the same polycarboxylate hydrogel used for every other chemistry, so an NTA sensor chip behaves like the rest of the range: four channels, 16 sensors, and the same regeneration workflow. Capture is oriented and reversible, which suits screening a series of constructs against one analyte without recoupling between runs.

Q: Can I order Biacore chips for the inQuiQ?

No. The inQuiQ uses its own photonic sensor chips rather than Biacore chips or other conventional SPR sensor chips. The chips carry a regenerable polycarboxylate hydrogel surface, with two surface types available for different measurement needs. Each chip provides 16 sensors, enabling multiplex measurements within a single run.

Every measurement on the inQuiQ happens on the biosensor chip. It carries your immobilized target, measures the injected sample, and returns the binding affinity in real time. Two biosensor chips are available, both regenerable, both built to limit non-specific binding in complex samples.

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01Surface chemistry

Two regenerable biosensor chips, matched to the binding question

Both chips use a polycarboxylate hydrogel over the photonic sensor. The difference is chain length, and it changes what the surface is good at. Pick by the measurement, not by the molecule.

HC1000M

Long hydrogel chains

Best for:

Low molecular weight analytes with small binding signals.
Concentration and quantification with standard curves.
Panel screening across multiple candidates.
Specificity testing with mismatch or off-target controls.

HC30M

Short hydrogel chains

Best for:

Affinity determination with clean equilibrium responses.
Full kinetic characterization.
Concentration series on a single immobilized target.
Sensorgram fit quality driving the interaction result.

Many programmes use both. Screen and confirm specificity on the HC1000M, then move the hits onto the HC30M for affinity and kinetics. If you are not sure where your assay sits, an application scientist will go through it with you before you order.

02Sample types

Immobilization chemistry designed around diverse samples

The inQuiQ measures binding affinity across molecule classes. Pick the coupling chemistry that fits your ligand, from EDC/NHS amine coupling to biotin, Strep-tag, Protein A/G, lipid layers, and click chemistry.

Sample compatibility

Diverse molecule types across one platform

Molecular scale

100 Da50 kDa150 kDaparticles

Surface chemistry

From a protein A sensor chip to click chemistry

Amine coupling

Covalent coupling to carboxyl groups via EDC/NHS

Streptavidin capture

Capture biotinylated ligands on a streptavidin or NeutrAvidin surface

Protein A and G capture

Immunoglobulin workflows

Streptactin and NTA

Capture Strep-tagged and his-tagged proteins

Lipid layers

Membrane proteins or lipid-interacting molecules

Azide groups

Click-based chemistry

03Chip + flowcell

Flexibly addressable flow channels

Four channels with customisable flow paths
4-plex assays with in-flow immobilization, up to 16-plex with a spotter
Antifouling hydrogel surface for clean signals in complex media
1 to 100 µL/min flow, up to 300 µL/min for low-viscosity samples

1–16 · sensor spots · pink · binding event

04inQuiQ specifications

Biosensor chip specifications

01 Sensors for complex samples

Antifouling layer keeps background signal low in complex samples.

02 Assay reproducibility

Regenerate the surface to test different samples with the same ligand.

03Consumable economics

Up to 16 different immobilized ligands on a spotted chip.

Sensor chip

Chip models

HC1000M, HC30M

Surface

Polycarboxylate hydrogel, antifouling

Channels

Sensors

164 per channel

Ligand spots per chip

4 in-flowup to 16 with a microarray spotter

Measurement range

Affinity, K_D

10 pM to 10 mM

Association rate, k_a

10¹ to 10⁷ M^-1s^-1

Dissociation rate, k_d

1 to 10^-6 s^-1

Sample refractive index range

Unlimited

Performance

Baseline noise

Typically < 0.1 RU RMSsensitivity chips Typically < 0.01 RU RMSat 1 Hz, high-sensitivity chips*
*Coming soon

Baseline drift

Typically < 0.3 RU/min

Flow rate range

1 to 100 µL/minup to 300 µL/min for low-viscosity samples

Sample and physical

Sample preparation

Unpurified samples after 0.22 µm filtration

Temperature range

16 to 40 °C4 °C below ambient

05Get a quote

Get a quote for inQuiQ sensor chips

Add the chips and quantities you need, and we will come back with a quote. If you are not sure which surface fits your target, an application scientist will go through it with you first.

Tell us what you need

Talk to an application scientist

Thirty minutes to discuss your assay design, before anyone mentions an instrument.

Download the inQuiQ brochure

Sensitivity, channel layout, footprint, and consumable formats in a couple of pages.

Delta Life Science
request@deltalifescience.nl
Send us an email, and we will get back to you as soon as possible.

Questions researchers ask

Both chips carry a polycarboxylate hydrogel over the photonic sensor. For users familiar with Biacore sensor chips, the practical distinction is similar: the surface is selected according to the measurement. The HC1000M uses long chains and suits low molecular weight analytes, quantification, screening, and specificity panels. The HC30M uses short chains and suits affinity determination and full kinetic characterization. Many programmes screen on one and confirm on the other.

Each chip has four channels with four sensors, 16 in total. Immobilized in flow, 4 different ligands can be immobilized on the chip. With a microarray spotter the sensors are addressed individually, giving up to 16 ligands for one measurement, including controls and reference surfaces.

Yes. The hydrogel surface is regenerable. After each injection the bound analyte is stripped and the surface returns to baseline, so one chip carries a full concentration series and keeps the same immobilized target in place across a measurement series.
Anyone used to regenerating an SPR sensor chip will recognize the workflow. Some immobilization techniques, such as Protein A/G, also allow the ligand to be stripped, enabling different ligands to be immobilized and measured sequentially on the same biosensor.

Yes. The antifouling hydrogel is built for serum, plasma, cell culture supernatant, and other complex matrices, where background binding usually limits the measurement. Filter the sample through a 0.22 µm filter and inject it directly. No purification step is needed first.

Yes. His-tagged ligands are captured on the same polycarboxylate hydrogel used for every other chemistry, so an NTA sensor chip behaves like the rest of the range: four channels, 16 sensors, and the same regeneration workflow. Capture is oriented and reversible, which suits screening a series of constructs against one analyte without recoupling between runs.

No. The inQuiQ uses its own photonic sensor chips rather than Biacore chips or other conventional SPR sensor chips. The chips carry a regenerable polycarboxylate hydrogel surface, with two surface types available for different measurement needs. Each chip provides 16 sensors, enabling multiplex measurements within a single run.