Knowledge hub
Practical guides for researchers who need to know whether and how strongly molecules bind. Start with the basics of label-free detection, learn how to set up and analyse a binding assay, compare methods, or read our take on new papers in your field.
Latest articles
New guides every week. Hover a card to read the summary.
ApplicationsSerum can raise, lower or blur the signal of a binding assay. Learn where matrix effects come from, how to test for them, and how to reduce them in ELISA and label-free assays.Matrix effects in binding assays: how serum changes your signalApplicationsTetraspanins CD9, CD63 and CD81 are the standard handles for detecting and capturing extracellular vesicles. This article explains what they are, why they are not on every vesicle, and how to set up binding measurements around them.Tetraspanins CD9, CD63 and CD81: EV markers and binding targetsAssay setupBaseline drift is often blamed on the chip. A short buffer-only test shows whether the surface, the buffer or the instrument is the real cause.Baseline drift in a biosensor: why it happens and how to stop itAssay setupHis-tag capture is fast and reversible, but the ligand keeps leaving the surface. How to measure that drift, keep it apart from the analyte interaction, and reduce it.His tag capture assay: what to check when capture levels driftApplicationsNanobodies often bind in the nanomolar to picomolar range. This guide covers which method to use, how to avoid avidity and off-rate traps, and how to check that a nanobody KD is reliable.How to measure nanobody binding affinity and kineticsAssay setupA square jump at the start of an injection is usually buffer, not binding. How to recognize a bulk shift, why the reference channel does not fully remove it, and how to match buffers.Bulk shift or binding? How to match buffers and read the jump
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Various topics, from first principles to the latest papers.
Assay setup
3 articlesPractical help for setting up a label-free binding assay. Immobilization, regeneration, buffers, controls and how to deal with non-specific binding.
Applications
3 articlesBinding analysis in your field, from engineered antibodies and small molecules to protein degraders, nucleic acids and lipid nanoparticles.
Need help with something else?
Tell an application specialist about your molecules and what you need to measure. You will get a straight answer on whether label-free analysis fits.