Assay setup
Practical help for setting up a label-free binding assay. Immobilization, regeneration, buffers, controls and how to deal with non-specific binding.
3 articles in Assay setup
Baseline drift in a biosensor: why it happens and how to stop itBaseline drift is often blamed on the chip. A short buffer-only test shows whether the surface, the buffer or the instrument is the real cause.
His tag capture assay: what to check when capture levels driftHis-tag capture is fast and reversible, but the ligand keeps leaving the surface. How to measure that drift, keep it apart from the analyte interaction, and reduce it.
Bulk shift or binding? How to match buffers and read the jumpA square jump at the start of an injection is usually buffer, not binding. How to recognize a bulk shift, why the reference channel does not fully remove it, and how to match buffers.Measured on inQuiQ
Application notes with real data from Delta's own lab.
WhitepaperReal-time, label-free NES analysis characterized PD-1/PD-L1 binding, delivering reproducible kinetic measurements with a KD of 37.7 nM.PD-1/PD-L1 InteractionWhitepaperLabel-free analysis quantified BMS-1166 inhibition of PD-1/PD-L1 binding, demonstrating dose-dependent inhibition with an IC₅₀ of 8.2 nM.PD-1/PD-L1 Inhibition with BMS-1166WhitepaperKinetic analysis of 250 Da small molecule interactions with Carbonic Anhydrase II using label-free sensing.Small Molecule Interactions
Have a question about this topic?
Tell an application specialist about your molecules and what you need to measure. You will get a straight answer on the setup that fits.